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Updated: Apr 19, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
Published on: January 17, 2015
Phage selection assisted by Sfp phosphopantetheinyl transferase-catalyzed site-specific protein labeling
Bo Zhao1, Keya Zhang, Karan Bhuripanyo
1Department of Chemistry, University of Chicago, 929 E57th Street, Chicago, IL, 60637, USA.
Abstract:
Phosphopantetheinyl transferases (PPTase) Sfp and AcpS catalyze a highly efficient reaction that conjugates chemical probes of diverse structures to proteins. PPTases have been widely used for site-specific protein labeling and live cell imaging of the target proteins. Here we describe the use of PPTase-catalyzed protein labeling in protein engineering by facilitating high-throughput phage selection.
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