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Updated: Apr 19, 2026
Cultivation Methods of Spirochetes from Borrelia burgdorferi Sensu Lato Complex and Relapsing Fever Borrelia
Published on: November 25, 2022
Long-term in vitro cultivation of Borrelia miyamotoi
Gabriele Margos1, Sylvia Stockmeier1, Cecilia Hizo-Teufel1
1Bavarian Health and Food Safety Authority, Oberschleissheim, Germany; German National Reference Centre for Borrelia, Oberschleissheim, Germany.
Abstract:
Borrelia are fastidious bacteria some of which are difficult to grow in vitro. Here, we report a method for successful continuous in vitro cultivation of the emerging pathogen Borrelia miyamotoi. The type and quantity of serum as well as the atmosphere were critical for successful in vitro cultivation. Optimal growth was achieved using 50% pooled human serum and an atmosphere of 6% CO2.
