Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Analysis of Tyre Pyrolysis Oil as Potential Diesel Fuel Blend with Focus on Swelling Behaviour of Nitrile-Butadiene Rubber.

Polymers·2025

Same author

Droplet-based cell viability assay for analysis of spheroid formation, proliferation and high-resolution IC<sub>50</sub> profiling.

Lab on a chip·2025

Same author

No effects of caffeine on cycling to exhaustion and perceptual responses in non-caffeine-restricted subjects.

Journal of the International Society of Sports Nutrition·2025

Same author

Bone Marrow Sparing by Intensity Modulated Proton Beam Therapy in Postoperative Irradiation of Gynecologic Malignancies.

Technology in cancer research & treatment·2024

Same author

Flexible Toolbox of High-Precision Microfluidic Modules for Versatile Droplet-Based Applications.

Micromachines·2024

Same author

Incidence and Risk Assessment of Capsular Contracture in Breast Cancer Patients following Post-Mastectomy Radiotherapy and Implant-Based Reconstruction.

Cancers·2024

Same journal

High-level De novo Biosynthesis of Plant-Derived Pinosylvin in Yarrowia lipolytica via Metabolic Engineering and Surfactant-Mediated Fermentation.

Journal of biotechnology·2026

Same journal

The effect of reduced graphene oxide in chitosan-based nanoparticles on the enzymatic properties of the immobilized enzyme.

Journal of biotechnology·2026

Same journal

High-level biosynthesis of gastrodin in engineered Escherichia coli.

Journal of biotechnology·2026

Same journal

From plasmid sequence to process design: A computational analysis of metabolism in the context of plasmid DNA manufacturing.

Journal of biotechnology·2026

Same journal

Development of an inducible cellobiohydrolase promoter and its application for enhancing the production of ganoderic acids in Ganoderma lingzhi.

Journal of biotechnology·2026

Same journal

Malic acid and allied exogenous chemicals induces desaturase gene expression and elevates PUFA production in marine microalgae Isochrysis sp.

Journal of biotechnology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 18, 2026

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

A modular segmented-flow platform for 3D cell cultivation.

Karen Lemke¹, Tobias Förster¹, Robert Römer¹

¹Department of Bioprocess Engineering, Institute for Bioprocessing and Analytical Measurement Techniques e.V., Rosenhof, D-37308 Heilbad Heiligenstadt, Germany.

Journal of Biotechnology

|January 7, 2015

Summary

This summary is machine-generated.

A new pipe-based bioreactor platform enables scalable 3D cell cultivation, mimicking in vivo conditions. This system supports stem cell self-assembly, proliferation, and differentiation for applications in regenerative medicine and drug testing.

Keywords:

Automated modular platform Droplets Embryoid body formation High(er) throughput Long-term 3D cell cultivation

More Related Videos

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

Published on: May 24, 2019

Author Spotlight: Developing a Unique Modular Microphysiological System to Mimic Human Barrier Tissue

Author Spotlight: Developing a Unique Modular Microphysiological System to Mimic Human Barrier Tissue

Published on: February 16, 2024

Related Experiment Videos

Last Updated: Apr 18, 2026

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

Published on: May 24, 2019

Author Spotlight: Developing a Unique Modular Microphysiological System to Mimic Human Barrier Tissue

Author Spotlight: Developing a Unique Modular Microphysiological System to Mimic Human Barrier Tissue

Published on: February 16, 2024

Area of Science:

Biotechnology
Cell Biology
Regenerative Medicine

Background:

In vitro 3D cell cultivation better mimics in vivo tissue than 2D cultures.
Realistic cell-matrix and cell-cell interactions are crucial for accurate biological modeling.
A scalable platform is needed to advance 3D cell culture applications.

Purpose of the Study:

To develop and validate a scalable 3D cell cultivation platform using pipe-based bioreactors (pbb).
To assess the platform's capability for stem cell self-assembly, proliferation, viability, and differentiation.
To explore the potential of pbb for long-term cell culture and personalized medicine applications.

Main Methods:

Development of a modular, automated pipe-based bioreactor (pbb) system utilizing segmented-flow technology.
Generation of micro-droplet bioreactors (60 nL to 20 μL) within a carrier fluid.
Utilized fluid management, storage, and analysis modules for high-throughput droplet generation and monitoring.

Main Results:

Demonstrated successful self-assembly of murine embryonic stem cells (mESCs) into uniform embryoid bodies (EBs).
Monitored cell proliferation, viability, and differentiation into cardiomyocytes within the pbb system.
Showcased the platform's ability to maintain cellular functions in a 3D microenvironment.

Conclusions:

The pipe-based bioreactor (pbb) platform offers a scalable and effective solution for in vitro 3D cell cultivation.
Pbb technology facilitates the study of stem cell behavior, including differentiation into cardiomyocytes.
The system holds promise for long-term cell culture, drug testing, and personalized medicine.