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Anti-complement sesquiterpenes from Viola yedoensis.

Dongsheng Du1, Zhihong Cheng1, Daofeng Chen1

  • 1Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, China.

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|January 7, 2015
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Summary

Two new compounds from Viola yedoensis, yedoensins A and B, show significant anti-complement activity. These germacrane sesquiterpenes modulate key complement pathway components, offering potential therapeutic insights.

Keywords:
Anti-complementMosher's methodSesquiterpenesViola yedoensis

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Area of Science:

  • Natural Products Chemistry
  • Immunology
  • Pharmacology

Background:

  • Viola yedoensis is a traditional medicinal herb.
  • Sesquiterpenes are a class of natural products with diverse biological activities.

Purpose of the Study:

  • To isolate and characterize new germacrane sesquiterpenes from Viola yedoensis.
  • To evaluate the anti-complement activity of isolated sesquiterpenes.
  • To investigate the mechanism of action of active compounds on the complement system.

Main Methods:

  • Isolation of compounds using chromatographic techniques.
  • Structure elucidation using 1D and 2D NMR, and HR-ESI-MS.
  • Determination of absolute configurations using a modified Mosher's method.
  • Assessment of anti-complement activity against classical (CP) and alternative (AP) pathways.
  • Mechanism of action studies using complement-depleted sera.

Main Results:

  • Two new germacrane sesquiterpenes, yedoensins A (1) and B (2), were identified.
  • Eight known sesquiterpenes (3-10) were also isolated.
  • Compounds 1-3 and 5-9 demonstrated anti-complement activity.
  • Specific compounds (1, 3, 6, 7, 9) were found to interact with complement components C1q, C3, C5, and C9.

Conclusions:

  • Viola yedoensis is a source of novel germacrane sesquiterpenes with anti-complement properties.
  • Yedoensins A and B, along with other isolated sesquiterpenes, represent potential modulators of the complement system.
  • Understanding the interactions with complement components provides a basis for developing new immunomodulatory agents.