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Evolutionary perspectives on multiresistance beta-lactamase transposons.

M Lafond¹, F Couture, G Vézina

¹Département de Microbiologie, Faculté de Médecine, Université Laval, Quebec, Canada.

Journal of Bacteriology

|December 1, 1989

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This study used DNA probes to analyze transposons, finding extensive homology between Tn3-like and Tn21-like elements. These findings suggest a distinct subfamily of mobile elements and propose an evolutionary model for multiresistance beta-lactamase transposons.

Area of Science:

Molecular Biology
Genetics
Microbiology

Background:

Transposons are mobile genetic elements that play a crucial role in genome evolution and the dissemination of antibiotic resistance.
Understanding the relationships between different transposon families is essential for tracking the spread of resistance genes.

Purpose of the Study:

To investigate the DNA sequence homology among various transposons, specifically Tn3, Tn21, and Tn2501.
To construct an evolutionary model for the development of multiresistance beta-lactamase transposons.

Main Methods:

Hybridization assays using intragenic DNA probes encoding transposase-resolvase and inverted repeats.
Southern-type gel electrophoresis to localize homologous sequences on physical DNA maps.
Comparison of genetic maps, physical maps, and nucleic acid sequence homologies.

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Main Results:

Extensive DNA homology was detected between Tn3-like and Tn21-like elements in 11 out of 18 transposons studied.
Homology was confirmed using specific inverted-repeat oligonucleotide probes.
Physical mapping localized transposase A (tnpA)-homologous sequences, aiding in comparative analysis.

Conclusions:

A distinct subfamily of mobile elements, related to the Tn21 group, was identified.
An evolutionary model and pedigree were proposed for the origin of multiresistance beta-lactamase transposons.