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Quantifying cell adhesion through impingement of a controlled microjet.

Claas Willem Visser¹, Marise V Gielen¹, Zhenxia Hao²

¹Physics of Fluids Group, MESA+ Institute, and J. M. Burgers Centre for Fluid Dynamics, University of Twente, Enschede, The Netherlands.

Biophysical Journal

|January 8, 2015

Summary

This summary is machine-generated.

This study models and measures shear stress from liquid jets to quantify cell adhesion. The jet impingement method assesses cell detachment and adhesion strength, showing promise for tissue engineering applications.

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Permeabilization of Adhered Cells Using an Inert Gas Jet

Permeabilization of Adhered Cells Using an Inert Gas Jet

Published on: September 4, 2013

Area of Science:

Biophysics
Cell Biology
Fluid Dynamics

Background:

Assessing cell attachment quantitatively in real-time is crucial for understanding cell adhesion.
Current methods using submerged liquid jets lack sufficient characterization for reliable use.

Purpose of the Study:

To model and measure shear stress from liquid jets on cell-covered surfaces.
To correlate shear stress with jet-induced cell detachment and quantify cell adhesion strength.

Main Methods:

Numerical modeling and experimental measurement of shear stress in the micrometer domain.
Real-time monitoring of cell detachment dynamics upon jet impingement.
Determination of cell adhesion strength using the jet impingement technique.

Main Results:

Measured and modeled shear stress data showed good agreement with previous studies.
Two cell detachment regimes were identified: dynamic and stationary.
A relationship between jet Reynolds number, cell-free area, and cell adhesion strength was established.
HeLa cell adhesion strength was determined to be (34 ± 14) N/m².
Cell detachment behavior (cell-by-cell vs. collective) was linked to cell monolayer density.

Conclusions:

The jet impingement method provides a reliable and quantitative approach for assessing cell adhesion.
This technique offers valuable insights into cell detachment mechanisms and adhesion strength.
The method holds significant potential for applications in tissue engineering and surface science.