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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
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Discovery and annotation of long noncoding RNAs
1Garvan Institute of Medical Research, Sydney, New South Wales, Australia, and St Vincent's Clinical School, University of New South Wales, Sydney, New South Wales, Australia.
Nature Structural & Molecular Biology
|January 8, 2015
Summary
Recent RNA sequencing has uncovered many long noncoding RNAs (lncRNAs) with unknown functions. This review outlines best practices for lncRNA identification and annotation to aid future research.
Area of Science:
- Genomics
- Molecular Biology
- Bioinformatics
Background:
- RNA sequencing advances reveal numerous unannotated noncoding transcripts.
- Long noncoding RNAs (lncRNAs) represent a significant portion of these discoveries.
- The functions of most identified lncRNAs are currently unknown.
Purpose of the Study:
- To discuss key considerations for lncRNA identification.
- To outline best practices for lncRNA annotation.
- To facilitate future functional and mechanistic studies of lncRNAs.
Main Methods:
- Review of current RNA sequencing methodologies.
- Analysis of established lncRNA identification pipelines.
- Discussion of annotation strategies and standards.
Main Results:
- Identification of challenges in lncRNA discovery and validation.
- Proposal of standardized approaches for lncRNA annotation.
- Highlighting the need for robust functional characterization.
Conclusions:
- Standardized identification and annotation are crucial for advancing lncRNA research.
- Best practices will accelerate the understanding of lncRNA functions.
- This work aims to guide researchers in exploring the lncRNA landscape.
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