Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

15.4K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
15.4K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

5.0K
5.0K
Types of RNA01:20

Types of RNA

16.8K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
16.8K
Types of RNA01:23

Types of RNA

74.3K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
74.3K
Translational Regulation01:29

Translational Regulation

885
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
885
Ribosome Profiling02:24

Ribosome Profiling

4.4K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inherited human TFIIIA deficiency disrupts T cell development.

medRxiv : the preprint server for health sciences·2026
Same author

Illuminating ribosome biogenesis disorders through structural biology.

RNA biology·2026
Same author

ADAR1 and ADAR2 associate with the RNA exosome and modulate RNA stability.

Nucleic acids research·2026
Same author

Mapping human pre-rRNA processing and modification at single nucleotide resolution using long read nanopore sequencing.

Nature communications·2026
Same author

Canine parvovirus infection affects host cell nucleolar organization and ribosome biogenesis.

Communications biology·2026
Same author

Intrinsically disordered regions stimulate concentration of small nucleolar ribonucleoproteins and formation of Cajal bodies and nucleoli.

Genes & development·2025

Related Experiment Video

Updated: Apr 18, 2026

Peering at Brain Polysomes with Atomic Force Microscopy
08:49

Peering at Brain Polysomes with Atomic Force Microscopy

Published on: March 16, 2016

8.9K

Noncoding RNAs in eukaryotic ribosome biogenesis and function.

Denis L J Lafontaine1

  • 1RNA Molecular Biology, Fonds National de la Recherche Scientifique, Université Libre de Bruxelles, Brussels, Belgium.

Nature Structural & Molecular Biology
|January 8, 2015
PubMed
Summary

Noncoding RNAs regulate ribosome biogenesis, impacting protein synthesis and cellular function. This review explores their roles in generating ribosome heterogeneity, affecting health and disease.

More Related Videos

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

24.9K
Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
10:56

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Published on: May 17, 2014

70.1K

Related Experiment Videos

Last Updated: Apr 18, 2026

Peering at Brain Polysomes with Atomic Force Microscopy
08:49

Peering at Brain Polysomes with Atomic Force Microscopy

Published on: March 16, 2016

8.9K
Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

24.9K
Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
10:56

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Published on: May 17, 2014

70.1K

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • The ribosome is essential for protein synthesis, with ribosomal RNA (rRNA) forming its functional core.
  • Ribosome biogenesis is a complex process involving numerous regulatory factors.
  • Noncoding RNAs play critical roles in regulating gene expression and cellular processes.

Purpose of the Study:

  • To review the diverse roles of noncoding RNAs in ribosome biogenesis.
  • To discuss how noncoding RNAs influence rRNA quantity and quality control.
  • To explore the contribution of noncoding RNAs to ribosome heterogeneity and its implications.

Main Methods:

  • Literature review of studies on noncoding RNAs and ribosome biogenesis.
  • Analysis of the mechanisms by which noncoding RNAs interact with rRNA.
  • Synthesis of current knowledge on ribosome heterogeneity and its functional consequences.

Main Results:

  • Noncoding RNAs are involved in multiple stages of ribosome biogenesis.
  • These RNAs modulate rRNA processing, folding, and assembly.
  • Noncoding RNAs contribute to the generation of functionally distinct ribosomes.

Conclusions:

  • Noncoding RNAs are key regulators of ribosome biogenesis and function.
  • Ribosome heterogeneity, influenced by noncoding RNAs, has significant implications for cellular processes.
  • Dysregulation of noncoding RNA-mediated ribosome control may contribute to disease states.