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Methodology for Sputum Induction and Laboratory Processing
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Sputum induction improves detection of pathogens in children with cystic fibrosis

Jordana E Hoppe1, Elinor Towler1, Brandie D Wagner2

  • 1Department of Pediatrics, Children's Hospital Colorado and the University of Colorado School of Medicine, Aurora, Colorado.

Pediatric Pulmonology
|January 8, 2015
PubMed

Insights

Sputum induction effectively diagnoses respiratory infections in children with cystic fibrosis (CF). This method improves pathogen detection compared to other sampling techniques, aiding in timely treatment adjustments for CF patients.

Area of Science:

  • Pediatric Pulmonology
  • Infectious Diseases
  • Clinical Microbiology

Background:

  • Sputum induction (IS) is a safe method for collecting lower airway secretions in children with cystic fibrosis (CF).
  • Its clinical utility in diagnosing outpatient infections requires further investigation.

Purpose of the Study:

  • To compare the success rate and microbiologic yield of induced sputum (IS) with oropharyngeal swabs (OP) and expectorated sputum (ES) in pediatric CF patients.
  • To determine if IS culture results influence antimicrobial treatment decisions.

Main Methods:

  • A prospective, longitudinal comparative study involving two cohorts of pediatric CF patients.
  • Simultaneous collection of OP, ES, and IS specimens in one cohort (n=17) over one year.
  • Comparison of IS culture results with the nearest respiratory culture within four months in the second cohort (n=35).

Main Results:

  • Sputum induction demonstrated a high success rate (94%) in outpatient pediatric CF encounters.
  • IS samples yielded a higher detection rate for polymicrobial infections, gram-negative organisms, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia compared to OP swabs.
  • The microbiologic yield of serial IS samples remained stable over one year.

Conclusions:

  • Sputum induction is a feasible and effective method for pathogen detection in the outpatient setting for children with CF.
  • IS improves the identification of respiratory pathogens, potentially enhancing clinical management strategies.
Abstract

Keywords:
Pseudomonas aeruginosaairway infectionchildrencystic fibrosissputum induction

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