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Updated: Jul 29, 2026

Particle Agglutination Method for Poliovirus Identification
Published on: April 20, 2011
Catecholamine-mediated inactivation of poliovirus RNA
1Department of Pathology and Microbiology, University of Nebraska, College of Medicine, Omaha 68105.
Abstract:
Catecholamine (DOPA, dopamine, epinephrine, or norepinephrine) and Cu2+ produced a potent inactivator(s) of transfectivity of poliovirus RNA. Al3+, Co2+, Fe3+, Mn2+, Ni2+, Sn4+ and Zn2+ did not substitute for Cu2+. Potency of inactivator(s) was eliminated by chelator (EDTA, histamine, or L-histidine). Kinetics of inactivation was single-hit, and inactivation was first order with respect to inactivator(s). A method for estimating relative concentration of inactivator(s) showed its course of production at 23 degrees C from norepinephrine at 10 microM and Cu2+ at 1.0 microM: under air, inactivator(s) peaked after 95 min, then decayed; under oxygen, peak was after 40 min; and under nitrogen, no production was detected. After prolonged exposure, most of the tritium from tritiated viral RNA appeared in small fragments. As tested with Cu2+ and norepinephrine, Al3+ and Fe3+ interfered strongly with production of inactivator(s) but not with preformed inactivator(s). An inactivator role for copper complexes of the semiquinone derivatives is hypothesized.
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