Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence and expression of a frog brain complementary DNA encoding a kainate-binding protein.

K Wada1, C J Dechesne, S Shimasaki

  • 1Laboratory of Molecular Otology, NIDCD, Bethesda, Maryland 20892.

Nature
|December 7, 1989
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Black Hole Spectroscopy and Tests of General Relativity with GW250114.

Physical review letters·2026
Same author

GW250114: Testing Hawking's Area Law and the Kerr Nature of Black Holes.

Physical review letters·2025
Same author

Search for Subsolar-Mass Binaries in the First Half of Advanced LIGO's and Advanced Virgo's Third Observing Run.

Physical review letters·2022
Same author

Simulated validation of intron-less transgene detection using DELLY for gene-doping control in horse sports.

Animal genetics·2021
Same author

Constraints on Cosmic Strings Using Data from the Third Advanced LIGO-Virgo Observing Run.

Physical review letters·2021
Same author

Prospects for observing and localizing gravitational-wave transients with Advanced LIGO, Advanced Virgo and KAGRA.

Living reviews in relativity·2020

Researchers identified the first molecular characterization of an excitatory amino acid (EAA) binding site by isolating a complementary DNA encoding a kainate-binding protein (KBP). This KBP may be a subunit of a kainate receptor-ionophore complex.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biochemistry

Background:

  • Excitatory amino acids (EAAs) are crucial neurotransmitters in the vertebrate central nervous system.
  • At least three EAA receptor subtypes (N-methyl-D-aspartate, kainate, and quisqualate) are known, distinguished by their preferred agonists.

Purpose of the Study:

  • To isolate and characterize the complementary DNA (cDNA) encoding a kainate-binding protein (KBP) from frog brain.
  • To determine if the KBP represents a molecular component of a kainate receptor-ionophore complex.

Main Methods:

  • Purification of KBP from frog brain using domoic acid affinity chromatography.
  • Isolation of KBP cDNA and deduction of its amino acid sequence.
  • Localization of KBP messenger RNA via in situ hybridization histochemistry.

Related Experiment Videos

  • Expression of KBP in COS-7 cells and assessment of kainate-binding activity.

    • Main Results:

    • A kainate-binding protein (Mr 48 K) was purified, exhibiting properties consistent with a kainate receptor-ionophore complex.
    • The deduced amino acid sequence of KBP shows similarities to ligand-gated ion channel subunits, including nicotinic acetylcholine receptors.
    • Transfected COS-7 cells displayed high-affinity kainate binding with pharmacological properties matching the purified KBP.

    Conclusions:

    • The study provides the first molecular characterization of an EAA binding site.
    • The isolated KBP cDNA likely encodes a ligand-binding subunit of a kainate receptor-ionophore complex.
    • This finding advances the understanding of excitatory amino acid receptor structure and function.