Ontogeny of strychnine-insensitive [3H]glycine binding sites in rat forebrain

The specific binding sites for strychnine-insensitive [3H]glycine were already demonstrable at prenatal stages and had increased to the adult level by postnatal day (PN) 10. This ontogenic increase was found to be due to an augmentation of Bmax without changes in kd of the [3H]glycine binding. Moreover, there was no shift in inhibition of the binding due to glycine, D-serine, L-serine and HA-966 (1-hydroxy-3-amino-pyrrolid-2-one) after birth. These findings are consistent with the view that there is an increase in density in the absence of the glycine recognition site associated with N-methyl-D-aspartate receptor/ion channel complex in rat forebrain during ontogenic development.