Related Experiment Video
Updated: Apr 18, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Cardiolipin signaling mechanisms: collapse of asymmetry and oxidation
Valerian E Kagan1,2,3,4, Yulia Y Tyurina1, Vladimir A Tyurin1
11Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, Pennsylvania.
Significance:
An ancient anionic phospholipid, cardiolipin (CL), ubiquitously present in prokaryotic and eukaryotic membranes, is essential for several structural and functional purposes.
Recent Advances:
The emerging role of CLs in signaling has become the focus of many studies.
Critical Issues:
In this work, we describe two major pathways through which mitochondrial CLs may fulfill the signaling functions via utilization of their (i) asymmetric distribution across membranes and translocations, leading to the surface externalization and (ii) ability to undergo oxidation reactions to yield the signature products recognizable by the executionary machinery of cells.
Future Directions:
We present a concept that CLs and their oxidation/hydrolysis products constitute a rich communication language utilized by mitochondria of eukaryotic cells for diversified regulation of cell physiology and metabolism as well as for inter-cellular interactions.
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