Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An investigation on protein and amino acid contents in scales and muscles of pomfret Parastromateus niger (Bloch, 1795) and Pampus argenteus (Eupharasen, 1788).

Brazilian journal of biology = Revista brasleira de biologia·2022

Same author

Effective water pollution control requires accurate data.

Environmental science & technology·2011

Same author

Crystal structure of the ectodomain of Methuselah, a Drosophila G protein-coupled receptor associated with extended lifespan.

Proceedings of the National Academy of Sciences of the United States of America·2001

Same author

Identification of a common variant in the lipoprotein lipase gene in a large Utah kindred ascertained for coronary heart disease: the -93G/D9N variant predisposes to low HDL-C/high triglycerides.

Clinical genetics·2001

Same author

Evidence of linkage of familial hypoalphalipoproteinemia to a novel locus on chromosome 11q23.

American journal of human genetics·2000

Same author

Genetic localization to chromosome 1p32 of the third locus for familial hypercholesterolemia in a Utah kindred.

Arteriosclerosis, thrombosis, and vascular biology·2000

Search research articles

Related Experiment Video

Updated: May 1, 2026

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

Published on: October 6, 2016

Targeted gene mutations in Drosophila.

D G Ballinger¹, S Benzer

¹Division of Biology, California Institute of Technology, Pasadena 91125.

Proceedings of the National Academy of Sciences of the United States of America

|December 1, 1989

Summary

This study introduces a novel "reverse genetics" method using polymerase chain reaction to detect transposable element insertions in specific genes. This technique facilitates the identification of gene function by observing mutant phenotypes in Drosophila.

More Related Videos

In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster

In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster

Published on: July 11, 2019

Author Spotlight: Optimizing Embryo Microinjection for Transgenesis in Drosophila

Author Spotlight: Optimizing Embryo Microinjection for Transgenesis in Drosophila

Published on: June 7, 2024

Related Experiment Videos

Last Updated: May 1, 2026

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

Published on: October 6, 2016

In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster

In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster

Published on: July 11, 2019

Author Spotlight: Optimizing Embryo Microinjection for Transgenesis in Drosophila

Author Spotlight: Optimizing Embryo Microinjection for Transgenesis in Drosophila

Published on: June 7, 2024

Area of Science:

Genetics
Molecular Biology
Developmental Biology

Background:

Identifying gene function often requires isolating mutations, which can be challenging.
Drosophila melanogaster is a key model organism for genetic studies.

Purpose of the Study:

To develop a general method for detecting transposable element insertions into cloned genes in Drosophila.
To enable the study of gene function through induced mutations and subsequent phenotype analysis.

Main Methods:

Utilized a "reverse genetics" approach involving transposable element insertion.
Employed polymerase chain reaction (PCR) with gene-specific and P-element primers to detect insertions.
Screened mutagenized fly populations for P-element insertions near a target gene.

Main Results:

Successfully detected single P-element transposon insertions within a specific proximity to a cloned gene.
Demonstrated the technique's efficacy in identifying insertions near a gene expressed in the Drosophila compound eye.

Conclusions:

The described PCR-based method is a sensitive tool for detecting transposable element insertions.
This approach provides a powerful means to link gene sequence to function by generating and analyzing mutant phenotypes.
The technique is adaptable for detecting insertions in various genes across different organisms.