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Updated: Apr 18, 2026
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Optical properties of tissues quantified using morphological granulometry from phase-contrast images of thin tissue
Zhifang Li1, Haiyu Chen2, Hui Li1
1Fujian Provincial Key Laboratory of Photonic Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, College of Photonic and Electronic Engineering, Fujian Normal University, Ministry of Education, Fuzhou, Fujian, China.
Abstract:
A method combining morphological granulometry with Mie theory to determine optical scattering in biological tissues was proposed. Otsu's method was applied to binarize phase-contrast images. Binary morphological granulometry was used to estimate size density distribution of the tissue samples based on the binary phase-contrast images. Our results showed that the optical parameters associated with light scattering in tissue could be quantitatively determined by combining size density distribution with Mie theory. It was suggested that this unique method could be used to characterize biological tissues for disease diagnosis.
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