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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Related Experiment Video

Updated: Apr 18, 2026

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

[Recent progress in single-cell RNA-Seq analysis].

Wen Lu¹, Tang Fuchou¹

¹Biodynamic Optical Imaging Center, College of Life Sciences, Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Peking University, Beijing 100871, China.

Yi Chuan = Hereditas

|January 9, 2015

Summary

This summary is machine-generated.

Single-cell RNA sequencing (scRNA-Seq) reveals gene expression in individual cells, overcoming limitations of traditional methods. This technology identifies diverse cell types and has expanded applications in developmental biology, immunology, and oncology.

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Last Updated: Apr 18, 2026

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

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Published on: January 7, 2020

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Published on: October 12, 2018

Area of Science:

Genomics
Molecular Biology
Biotechnology

Context:

Biological tissues exhibit inherent cell heterogeneity.
Traditional transcriptome analysis averages gene expression, masking cellular diversity.
Single-cell RNA sequencing (scRNA-Seq) enables gene expression profiling at the individual cell level.

Purpose:

To review recent advancements in the methodology of single-cell RNA sequencing (scRNA-Seq).
To highlight the expanding applications of scRNA-Seq across various biological fields.
To underscore the importance of scRNA-Seq in understanding cell heterogeneity.

Summary:

scRNA-Seq technologies have rapidly advanced, offering new cDNA amplification methods and quantitative analyses of sensitivity and noise.
Developments include low-coverage high-throughput and in situ scRNA-Seq.
Applications now span embryonic and organ development, immunology, and oncology.

Impact:

Enables precise identification of distinct cell types within heterogeneous populations.
Provides deeper insights into developmental processes and disease mechanisms.
Drives innovation in fields like cancer research and immunology by dissecting cellular composition.