Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Eicosanoid production in rheumatoid synovitis.

E Moilanen1, J Alanko, M Nissilä

  • 1Department of Biomedical Sciences, University of Tampere, Finland.

Agents and Actions
|November 1, 1989
PubMed
Summary

Rheumatoid arthritis patients show increased leukotriene B4 (LTB4) synthesis in synovial fluid polymorphonuclear leukocytes (PMNs). Synovial lining cells primarily produce prostaglandin E2 (PGE2), not LTB4, in inflamed joints.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The biological activity of serum bacterial lipopolysaccharides associates with disease activity and likelihood of achieving remission in patients with rheumatoid arthritis.

Arthritis research & therapy·2022
Same author

Pretreatment resistin levels are associated with erosive disease in early rheumatoid arthritis treated with disease-modifying anti-rheumatic drugs and infliximab.

Scandinavian journal of rheumatology·2021
Same author

Induction of remission in female rheumatoid arthritis patients is associated with stabilization of myocardial abnormalities: a prospective cardiac magnetic resonance follow-up study.

Scandinavian journal of rheumatology·2020
Same author

OARSI guidelines for the non-surgical management of knee, hip, and polyarticular osteoarthritis.

Osteoarthritis and cartilage·2019
Same author

Aprotinin Impacts 8-Isoprostane after Coronary Artery Bypass Grafting.

Scandinavian journal of surgery : SJS : official organ for the Finnish Surgical Society and the Scandinavian Surgical Society·2018
Same author

Signalling Profiles of Blood Leucocytes in Sepsis and in Acute Pancreatitis in Relation to Disease Severity.

Scandinavian journal of immunology·2017

Area of Science:

  • Immunology
  • Rheumatology
  • Biochemistry

Background:

  • Rheumatoid synovitis involves complex inflammatory processes.
  • Leukotriene B4 (LTB4) and prostaglandin E2 (PGE2) are key inflammatory mediators.
  • Understanding the cellular sources of these mediators is crucial for targeted therapies.

Purpose of the Study:

  • To investigate the synthesis of LTB4 in rheumatoid synovitis.
  • To compare LTB4 production by peripheral and synovial polymorphonuclear leukocytes (PMNs).
  • To determine the role of synovial lining cells in LTB4 and PGE2 production.

Main Methods:

  • Collected peripheral and synovial fluid PMNs from healthy volunteers and rheumatoid arthritis (RA) patients.
  • Cultured rheumatic synovial lining cells.

Related Experiment Videos

  • Stimulated cells with arachidonic acid and Ca ionophore A23187 to measure LTB4 and PGE2 synthesis.

    • Main Results:

    • No difference in LTB4 synthesis between peripheral PMNs from healthy and RA individuals.
    • Synovial fluid PMNs from RA patients showed significantly increased arachidonic acid-induced LTB4 synthesis compared to peripheral PMNs.
    • Synovial lining cells produced minimal LTB4 but significant amounts of PGE2, with LTB4 production unaffected by stimuli.

    Conclusions:

    • LTB4 in inflamed joints primarily originates from PMNs, not synovial lining cells.
    • Synovial lining cells are a major source of PGE2 in rheumatoid synovitis.
    • The findings suggest localized factors in inflamed joints enhance PMN LTB4 synthesis.