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Evidence for tight metabolic control of the receptor-activated polyphosphoinositide cycle in human platelets
V M Steen1, O B Tysnes, H Holmsen
1Department of Biochemistry, University of Bergen, Norway.
The Biochemical Journal
|October 15, 1989
Abstract:
The [32P]PIP2/[32P]PA and the [32P]PIP/[32P]PA relationships were demonstrated to be remarkably similar after stimulation of [32P]Pi-prelabelled platelets for 90 s with various combinations and concentrations of agonists and inhibitors. Thus the activity of the PI and PIP kinases with the corresponding phosphomonoesterases may be tightly controlled during receptor-mediated platelet stimulation involving phospholipase C activation.