Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Apr 18, 2026

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly
10:17

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly

Published on: November 4, 2021

3.8K

Complex micropatterning of proteins within microfluidic channels.

Miju Kim, Junsang Doh

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
    |January 9, 2015
    PubMed
    Summary

    Researchers developed a novel method for integrating microfluidics and protein micropatterning. This technique uses poly(dimethylsiloxane) channels and a bio-friendly photoresist to create intricate protein patterns within microfluidic devices.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Redispersible nanoalum-adjuvanted hepatitis B vaccine microneedles with enhanced immunogenicity.

    Drug delivery and translational research·2026
    Same author

    Enhanced stability and sustained delivery of structurally dense DNA nanostructures <i>via</i> a biodegradable hydrogel platform.

    Nanoscale horizons·2026
    Same author

    Engineering T Cells with Membrane-Anchored Nitric Oxide Scavengers for Anticancer Therapy.

    ACS nano·2026
    Same author

    Jammed Foamed Microgel-based Bioprinting for Ex Vivo Reconstruction of 3D T Cell-Cancer Cell Interactions.

    Advanced healthcare materials·2025
    Same author

    Tumor-priming CD8<sup>+</sup> natural killer T-like cells as an efficient novel cell therapy for relapsed/refractory multiple myeloma.

    Experimental hematology & oncology·2025
    Same author

    Deep sequencing of Korean Jindo dog reveals evolutionary trajectory of coat color variations.

    Genomics·2025

    Area of Science:

    • Biotechnology
    • Materials Science
    • Analytical Chemistry

    Background:

    • Microfluidic channels with patterned protein surfaces are crucial for bioanalytical and biological applications.
    • Existing methods for protein micropatterning in microfluidics face challenges in integration and biocompatibility.

    Purpose of the Study:

    • To develop a novel, integrated method for creating protein micropatterns within microfluidic channels.
    • To utilize a bio-friendly photoresist and an adhesive layer for robust microchannel fabrication.

    Main Methods:

    • Synthesized a novel bio-friendly photoresist: poly(2,2-dimethoxy nitrobenzyl methacrylate-r-methyl methacrylate-r-poly(ethylene glycol) methacrylate) (PDMP).
    • Utilized poly(dopamine) (PDA) as an adhesive to bond poly(dimethylsiloxane) (PDMS) microfluidic channels to PDMP films.

    More Related Videos

    A Versatile Method of Patterning Proteins and Cells
    09:57

    A Versatile Method of Patterning Proteins and Cells

    Published on: February 26, 2017

    9.9K
    Microcontact Printing of Proteins for Cell Biology
    09:21

    Microcontact Printing of Proteins for Cell Biology

    Published on: December 5, 2008

    21.8K

    Related Experiment Videos

    Last Updated: Apr 18, 2026

    Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly
    10:17

    Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly

    Published on: November 4, 2021

    3.8K
    A Versatile Method of Patterning Proteins and Cells
    09:57

    A Versatile Method of Patterning Proteins and Cells

    Published on: February 26, 2017

    9.9K
    Microcontact Printing of Proteins for Cell Biology
    09:21

    Microcontact Printing of Proteins for Cell Biology

    Published on: December 5, 2008

    21.8K
  • Employed microscope projection photolithography (MPP) to pattern proteins directly within the microfluidic channels on the PDMP surface.

    • Main Results:

    • Successfully integrated PDMS microfluidic channels with PDMP photoresist films using PDA adhesive.
    • Demonstrated the capability to generate complex protein micropatterns directly within the microfluidic channels.
    • Validated the bio-friendly nature of the PDMP photoresist for microfluidic applications.

    Conclusions:

    • The developed method offers a facile and effective approach for creating protein micropatterned microfluidic devices.
    • This technique enhances the utility of microfluidics in various bioanalytical and biological applications requiring precise protein localization.
    • The use of bio-friendly materials and integrated patterning advances microfluidic device fabrication.