Does insulin impact cold-induced fluid- and protein-extravasation?
Hege K Brekke1, Stig Hammersborg1, Torbjørn Nedrebø2
1Department of Anesthesia and Intensive Care, Haukeland University Hospital, 5021 Bergen, Norway.
Cryobiology
|January 10, 2015
Summary
Insulin did not significantly alter fluid and protein leakage during hypothermia in animal studies. This suggests insulin
Area of Science:
- Physiology
- Endocrinology
- Vascular Biology
Background:
- Insulin stabilizes the endothelial barrier by affecting cell adhesion and contractility.
- Hypothermia can increase fluid and protein leakage from blood vessels.
- The anti-inflammatory and endothelial-stabilizing effects of insulin were investigated in the context of hypothermia.
Purpose of the Study:
- To determine if insulin administration influences fluid and protein extravasation during hypothermia.
- To assess the impact of insulin on endothelial barrier function under hypothermic conditions.
Main Methods:
- A randomized controlled study involving two groups of animals cooled to 28°C.
- One group received insulin infusion (I-group), while the control group (C-group) did not.
- Measurements included fluid balance, hemodynamics, plasma volume, colloid osmotic pressures, hematocrit, cytokine profiles, and protein concentrations to calculate fluid extravasation rate (FER).
Main Results:
- Albumin and protein masses, along with plasma colloid osmotic pressure (COPp), decreased significantly in both groups during hypothermia.
- Plasma volume decreased significantly in the control group but showed only a decreasing trend in the insulin group.
- Fluid extravasation rate (FER) showed a slight increasing trend in both groups, with no significant differences between the insulin and control groups.
Conclusions:
- Insulin administration did not significantly affect fluid and protein extravasation during prolonged hypothermia in this animal model.
- The endothelial-stabilizing properties of insulin did not prevent increased vascular leakage under hypothermic conditions.
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