Related Experiment Videos
Cloning in Escherichia coli of the enterotoxin gene from Clostridium perfringens type A
L A Iwanejko1, M N Routledge, G S Stewart
1Department of Applied Biochemistry and Food Science, University of Nottingham, Sutton Bonington, Leicestershire, UK.
Abstract:
A 26 bp DNA probe has been constructed with minimal degeneracy to the protein sequence for Clostridium perfringens enterotoxin. The probe has been hybridized against a 6-10 kb chromosomal bank from C. perfringens 8239, prepared as a HindIII partial digest in pHG165. From this survey a clone has been identified containing a 6.8 kb DNA insert with strong hybridization to the probe. Direct plasmid sequencing has identified a translational reading frame within this clone which correlates with the known protein sequence for the type A enterotoxin. DNA sequences 5' to this open reading frame and containing the putative transcriptional control regions show areas of significant homology with regions upstream from the ATG codon of the tetanus toxin gene.