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Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

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Enhanced quantitative phase imaging in self-interference digital holographic microscopy using an electrically focus

Robin Schubert¹, Angelika Vollmer², Steffi Ketelhut³

¹Center for Biomedical Optics and Photonics, University of Muenster, Robert-Koch-Str. 45, D-48149 Muenster, Germany ; George Huntington Institute, Johann-Krane-Weg 27, D-48149 Muenster, Germany.

Biomedical Optics Express

|January 10, 2015

Summary

This study introduces a new method to reduce laser light scattering in digital holographic microscopy (DHM) for clearer imaging of living cells. The technique significantly improves quantitative phase imaging by minimizing coherent disturbances.

Keywords:

(090.1995) Digital holography (110.0180) Microscopy (110.1650) Coherence imaging (120.5050) Phase measurement (170.1530) Cell analysis

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Area of Science:

Biomedical Optics
Microscopy
Cell Biology

Background:

Self-interference digital holographic microscopy (DHM) is suitable for quantitative phase imaging of living cells.
Coherent laser light in DHM causes scattering patterns, reducing resolution for optical path length changes.

Purpose of the Study:

To present a simple and efficient technique for reducing coherent disturbances in quantitative phase images.
To enhance the resolution and accuracy of self-interference DHM for biological samples.

Main Methods:

Modulating the amplitude and phase of sample illumination using an electrically focus tunable lens.
Implementing this modulation within the self-interference DHM setup.

Main Results:

Achieved a reduction of coherence-induced disturbances by up to 70%.
Demonstrated enhanced quantitative imaging performance for living cells.

Conclusions:

The proposed method effectively reduces coherent disturbances in self-interference DHM.
This technique offers improved quantitative phase imaging for live-cell studies.