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A high-throughput screening method for determining the substrate scope of nitrilases
Gary W Black1, Nicola L Brown, Justin J B Perry
1Industrial Biotechnology Research Group, Department of Applied Sciences, Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne, NE1 8ST, UK. justin.perry@northumbria.ac.uk.
Chemical Communications (Cambridge, England)
|January 10, 2015
Abstract:
Nitrile compounds are intermediates in the synthesis of pharmaceuticals such as atorvastatin. We have developed a chromogenic reagent to screen for nitrilase activity as an alternative to Nessler's reagent. It produces a semi-quantifiable blue colour and hydrolysis of 38 nitrile substrates by 23 nitrilases as cell-free extracts has been shown.
