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Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
Published on: May 6, 2022
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DNA methylation regulates bromodomain-containing protein 2 expression during adipocyte differentiation
Ruixin Sun1, Yi Wu, Yuxiong Wang
1The Key Laboratory of Molecular Medicine, The Ministry of Education, Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, P.O. Box #238, No. 138 Yi Xue Yuan Road, Shanghai, 200032, China.
Molecular and Cellular Biochemistry
|January 11, 2015
Summary
DNA methylation regulates Bromodomain-containing protein 2 (Brd2) expression during adipocyte differentiation. Demethylation of the Brd2 promoter increases Brd2 expression, impacting fat cell development.
Area of Science:
- Molecular Biology
- Epigenetics
- Cell Biology
Background:
- Obesity involves excessive white adipose tissue accumulation.
- Bromodomain-containing protein 2 (Brd2) inhibits adipocyte differentiation.
- DNA methylation's role in adipocyte differentiation is not fully understood.
Purpose of the Study:
- To investigate if DNA methylation regulates Brd2 expression during adipocyte differentiation.
- To elucidate the mechanism by which Brd2 expression is controlled epigenetically.
Main Methods:
- 3T3-L1 pre-adipocytes were treated with the methylation inhibitor 5-aza-2'-deoxycytidine (5-Aza).
- Brd2 mRNA and protein levels were quantified.
- Bisulfite sequencing and BstU1 digestion analyzed Brd2 promoter DNA methylation status.
Main Results:
- 5-Aza treatment increased Brd2 transcription in pre-adipocytes and adipocytes.
- Mature adipocytes showed increased Brd2 protein expression after 5-Aza treatment.
- Brd2 promoter demethylation correlated with increased Brd2 mRNA levels during differentiation.
Conclusions:
- DNA demethylation of the Brd2 promoter is a key mechanism regulating its expression.
- Epigenetic regulation of Brd2 influences adipocyte differentiation.
- Findings provide insights into the molecular basis of adipogenesis.
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