Related Experiment Video

Updated: Apr 18, 2026

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
12:49

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

10.6K

DICER-dependent biogenesis of let-7 miRNAs affects human cell response to DNA damage via targeting p21/p27

Bailong Liu1, Min Liu1, Jian Wang2

  • 1Department of Radiation Oncology, The First Hospital of Jilin University, Changchun 130021, China Department of Radiation Oncology, Emory University School of Medicine and Winship Cancer Institute, Atlanta, GA 30322, USA.

Nucleic Acids Research
|January 13, 2015
PubMed

Insights

Knockdown of DICER enhances cell resistance to DNA damage by prolonging G1/S transition, affecting microRNA let-7 levels and DNA repair efficiency. This study reveals DICER

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • DICER is involved in small RNA generation and DNA damage response.
  • Previous studies suggested DICER knockdown impairs DNA repair.

Purpose of the Study:

  • To investigate the role of DICER in cellular response to DNA damage.
  • To elucidate the mechanism by which DICER affects DNA repair and cell cycle progression.

Main Methods:

  • Cell culture and treatment with camptothecin.
  • Western blotting to assess protein levels (p21, p27).
  • Analysis of cell cycle progression and DNA repair efficiency.

Main Results:

  • DICER knockdown increased resistance to camptothecin-induced DNA damage.
  • This resistance was linked to a prolonged G1/S transition due to decreased microRNA let-7 biogenesis.
  • Reduced microRNA let-7 led to increased p21(Waf1/Cip1)/p27(Kip1) levels, impairing homologous recombination repair (HRR).

Conclusions:

  • DICER plays a novel role in regulating cell cycle progression via microRNA biogenesis.
  • This regulation impacts cellular responses to DNA damage and repair mechanisms.
  • The findings challenge previous understandings of DICER's function in DNA damage response.

More Related Videos

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

8.1K
Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

14.8K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.4K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
25.1K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.4K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.4K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
39.2K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
6.3K