Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioreactor Controls-III01:22

Bioreactor Controls-III

59
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
59
Evolution of New Traits in Microbes01:24

Evolution of New Traits in Microbes

184
Microorganisms evolve rapidly due to their large population sizes and short generation times, often exhibiting measurable changes within days under laboratory conditions. Natural selection acts on standing genetic variation, enabling the retention and amplification of beneficial traits that confer fitness advantages in changing environments.Adaptive Pigment Regulation in RhodobacterIn Rhodobacter, a genus of purple non-sulfur bacteria, light-harvesting pigments such as bacteriochlorophyll and...
184
Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

5.5K
The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
 
Most enzymes...
5.5K
Upstream Processing01:27

Upstream Processing

92
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
92
Microbial Bioremediation of Plastics01:28

Microbial Bioremediation of Plastics

108
Polyethylene terephthalate (PET) is a synthetic polymer widely utilized in the packaging industry, particularly for bottles and containers. Due to its chemical stability and durability, PET accumulates in the environment, contributing significantly to plastic pollution. It comprises repeating units of terephthalic acid and ethylene glycol, resulting in a semi-crystalline structure that is resistant to natural degradation processes.A notable breakthrough in plastic biodegradation came with the...
108
Biosynthesis in Bacteria01:24

Biosynthesis in Bacteria

1.0K
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
1.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The expanding role of protease therapeutics (2012-2026): from replacement therapies to immune system modulation and beyond.

The Biochemical journal·2026
Same author

A Versatile Enzymatic Pathway for Modification of Peptide C‑Termini.

ACS central science·2025
Same author

High-Throughput Activity Reprogramming of Proteases (HARP).

ACS chemical biology·2025
Same author

A versatile enzymatic pathway for modification of peptide C-termini.

bioRxiv : the preprint server for biology·2025
Same author

Genome mining of RiPPs driven by highly efficient pathway reconstruction methods.

Methods in enzymology·2025
Same author

Protease engineering: Approaches, tools, and emerging trends.

Biotechnology advances·2025

Related Experiment Video

Updated: Apr 18, 2026

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

18.7K

Improving and repurposing biocatalysts via directed evolution.

Carl A Denard1, Hengqian Ren1, Huimin Zhao2

  • 1Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States.

Current Opinion in Chemical Biology
|January 13, 2015
PubMed
Summary

Directed evolution enhances biocatalyst performance and creates novel enzymes. Recent advances focus on improving thermostability, stereoselectivity, and activity for industrial applications.

More Related Videos

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

11.5K
Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
09:27

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

18.4K

Related Experiment Videos

Last Updated: Apr 18, 2026

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

18.7K
Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

11.5K
Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
09:27

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

18.4K

Area of Science:

  • Biochemistry
  • Protein Engineering
  • Industrial Biotechnology

Background:

  • Directed evolution is a cornerstone of modern protein engineering.
  • It has revolutionized industrial biocatalysis by enabling enzyme optimization.
  • This technique allows for the development of biocatalysts with enhanced or novel functionalities.

Purpose of the Study:

  • To review recent advancements in directed evolution over the past two years.
  • To highlight successes in improving biocatalyst properties.
  • To discuss the creation of novel enzymes through directed evolution.

Main Methods:

  • Directed evolution techniques applied to protein engineering.
  • Focus on enhancing specific enzyme characteristics.
  • Development of biocatalysts with new chemical reactivities.

Main Results:

  • Significant improvements in biocatalyst thermostability achieved.
  • Enhanced stereoselectivity demonstrated in engineered enzymes.
  • Increased activity and novel catalytic functions engineered into biocatalysts.
  • Recent successes in creating entirely novel enzymes.

Conclusions:

  • Directed evolution continues to drive innovation in biocatalysis.
  • Recent progress demonstrates its power in tailoring enzyme properties.
  • The field is expanding the scope of biocatalysis into new chemical territories.