Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The F1000Research Antibody Validation Article Collection.

Matthew A Helsby1, Mei Yee Leung2, Andrew D Chalmers3

  • 1CiteAb Limited, Bath, BA1 1BE, UK.

F1000Research
|January 20, 2015
PubMed
Summary

Antibody validation is essential for life science research. This collection encourages publishing all antibody validation studies, positive or negative, to prevent duplicated efforts and improve antibody reliability.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Antibody Society's antibody validation webinar series.

mAbs·2020
Same author

Cell surface dynamics and cellular distribution of endogenous FcRn.

PloS one·2017
Same author

A subset of high Gleason grade prostate carcinomas contain a large burden of prostate cancer syndecan-1 positive stromal cells.

The Prostate·2017
Same author

Truncated RASSF7 promotes centrosomal defects and cell death.

Developmental biology·2015
Same author

Correction: Formation of a Polarised Primitive Endoderm Layer in Embryoid Bodies Requires Fgfr/Erk Signalling.

PloS one·2015
Same author

Formation of a polarised primitive endoderm layer in embryoid bodies requires fgfr/erk signalling.

PloS one·2014

Area of Science:

  • Life sciences
  • Biotechnology
  • Immunology

Background:

  • Validated antibodies are critical for reproducible life science research.
  • Antibody validation is a complex, often unpublished, preliminary step.
  • Duplication of effort is common due to unpublished validation data.

Purpose of the Study:

  • To establish a dedicated collection for antibody validation studies.
  • To encourage the publication of all validation results, including negative findings.
  • To create a valuable resource for researchers using antibodies.

Main Methods:

  • Publication of antibody validation studies of varying scales.
  • Inclusion of studies validating individual antibodies or large antibody sets.
  • Acceptance of opinion and correspondence articles on antibody validation.

Related Experiment Videos

Main Results:

  • A new collection for antibody validation research has been established.
  • Encourages sharing of both positive and negative antibody performance data.
  • Aims to reduce redundant research efforts in antibody validation.

Conclusions:

  • The F1000Research collection will serve as a vital resource for antibody validation.
  • Promoting open publication of validation data enhances research reproducibility.
  • Standardized validation reporting benefits the scientific community.