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Related Concept Videos

Proteomics01:33

Proteomics

10.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.2K

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Related Experiment Video

Updated: Apr 18, 2026

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
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Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

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Using tissue samples for proteomic studies-critical considerations.

Karl-Friedrich Becker1

  • 1Institute of Pathology, Technische Universität München, München, Germany.

Proteomics. Clinical Applications
|January 13, 2015
PubMed
Summary
This summary is machine-generated.

Tissue sample quality is critical for proteomic analysis. Both frozen and formalin-fixed tissues present preanalytical challenges that impact results, necessitating workflow standardization for clinical translation.

Keywords:
BiomarkerCancerPreanalytical phaseReverse phase protein arrayTissue

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Area of Science:

  • Proteomics
  • Pathology
  • Biochemistry

Background:

  • Proteomic analysis of human tissues is vital for clinical research.
  • Researchers often overlook preanalytical variables affecting tissue sample quality.
  • Both frozen and formalin-fixed tissues present unique challenges for proteomic studies.

Purpose of the Study:

  • To review the progress of proteomic studies using human tissues.
  • To highlight critical preanalytical challenges in tissue sample acquisition and processing.
  • To emphasize the need for standardization in proteomic workflows for clinical translation.

Main Methods:

  • Review of existing literature on proteomic analysis of human tissues.
  • Discussion of preanalytical factors impacting frozen and formalin-fixed tissue samples.
  • Analysis of challenges in translating proteomic methods to clinical practice.

Main Results:

  • Formalin fixation introduces cross-linking, altering protein profiles.
  • Frozen tissue samples can also degrade or be compromised, affecting proteomic data.
  • Lack of awareness regarding preanalytical issues is widespread among researchers.
  • Protein profiles can change significantly due to sample processing before analysis.

Conclusions:

  • Standardization of the entire workflow, especially the preanalytical phase, is crucial for reliable proteomic results.
  • Addressing preanalytical challenges is essential for the successful clinical integration of proteomic studies.
  • Researchers must critically evaluate tissue sample quality alongside analytical techniques.