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Comparison of cryopreservation bags for hematopoietic progenitor cells using a WBC-enriched product
Margriet J Dijkstra-Tiekstra1, Sandra Hazelaar1, Effimia Gkoumassi1
1Sanquin Blood Supply, Department Transfusion Monitoring, Groningen, The Netherlands.
Abstract:
Hematopoietic progenitor cells (HPC) are stored in cryopreservation bags that are resistant to liquid nitrogen. Since Cryocyte bags of Baxter (B-bags) are no longer available, an alternative bag was sought. Also, the influence of freezing volume was studied. Miltenyi Biotec (MB)- and MacoPharma (MP)-bags passed the integrity tests without failure. Comparing MB- and MP-bags with B-bags, no difference in WBC recovery or viability was found when using a WBC-enriched product as a "dummy" HPC product. Further, a freezing volume of 30 mL resulted in better WBC recovery and viability than 60 mL. Additonal studies using real HPC might be necessary.
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