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Updated: Apr 18, 2026
Human Egg Maturity Assessment and Its Clinical Application
Published on: August 19, 2019
Confocal microscopic analysis of the microfilament configurations from human vitrification-thawed oocytes matured in
Qianqian Ci1, Mei Li1, Yingchun Zhang2
1Center for Reproductive Medicine, Shandong Provincial Hospital, Shandong University, Jinan, China.
Background:
The alteration in microfilaments of human oocytes by vitrification has not been understood well.
Objective:
To evaluate the effect of vitrification on the microfilaments of human in vitro matured oocytes and the time needed for the repair.
Materials And Methods:
Human in vitro matured oocytes were divided into the control group (Group 1) and the vitrified group. The vitrified oocytes were further divided into four sub-groups that were cultured after thawing for 1 h, 2 h, 3 h and 4 h, respectively (Groups 2, 3, 4 and 5).
Results:
The survival rate of oocytes was 87.4% after vitrification and thawing. The percentage of oocytes with a normal configuration of microfilaments in Group 2 and Group 3 was significantly lower than that of the fresh control group (Group 1), whereas the percentage of oocytes with normal microfilament configuration in Group 4 and Group 5 was comparable to the control group.
Conclusion:
Vitrification alters microfilament structure of oocytes, which takes at least 3h after thawing for the repair and recovery.
