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Updated: Apr 18, 2026

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
Published on: October 20, 2020
[Proteomics in infectious diseases]
Sara Quero1, Noemí Párraga-Niño2, Marian García-Núñez3
1Unitat de Malalties Infeccioses, Fundació Institut d'Investigació Germans Trias i Pujol, Badalona, Barcelona, España; Universitat Autònoma de Barcelona, Cerdanyola, Barcelona, España.
Abstract:
Infectious diseases have a high incidence in the population, causing a major impact on global health. In vitro culture of microorganisms is the first technique applied for infection diagnosis which is laborious and time consuming. In recent decades, efforts have been focused on the applicability of "Omics" sciences, highlighting the progress provided by proteomic techniques in the field of infectious diseases. This review describes the management, processing and analysis of biological samples for proteomic research.
Insights
Proteomics offers a faster alternative to traditional microorganism culture for diagnosing infectious diseases. This review details sample handling and analysis for proteomic research in this field.
Area of Science:
- Microbiology
- Proteomics
- Infectious Diseases
Background:
- Infectious diseases significantly impact global health.
- Traditional in vitro microorganism culture for diagnosis is time-consuming and labor-intensive.
- Omics sciences, particularly proteomics, show promise for advancing infectious disease research.
Purpose of the Study:
- To review the application of proteomics in infectious disease research.
- To describe methods for managing, processing, and analyzing biological samples for proteomic studies.
Main Methods:
- Literature review of proteomic techniques in infectious disease diagnosis.
- Discussion of biological sample preparation for proteomic analysis.
- Overview of data processing and interpretation in proteomic research.
Main Results:
- Proteomics provides a more efficient approach compared to conventional diagnostic methods.
- Standardized protocols for sample management and analysis are crucial for reliable proteomic data.
- Proteomic strategies can accelerate the diagnosis and understanding of infectious agents.
Conclusions:
- Proteomics is a valuable tool for the rapid diagnosis and study of infectious diseases.
- Effective sample management and analysis are key to leveraging proteomics in this domain.
- Further integration of proteomic techniques will enhance global health strategies against infections.
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