[Production, purification and evaluation of human cytomegalovirus 66 kD structural antigen for antibody detection by ELISA]
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P Gireaudot1, A Dewilde, P E Lobert
1Service de Bactériologie Virologie B, Centre Hospitalier Regional, IRFPPS, Lille, France.
Pathologie-Biologie
|December 1, 1989
Abstract:
An ELISA method using human CMV major polypeptide (66 kD) purified by electroelution was used to detect IgG and IgM antibodies and was compared with a conventional ELISA using the whole virus. The ELISA-66 kD showed a good sensitivity (98.2%) and had a better specificity than the ELISA-whole virus (97.5% versus 80.3%). Cross reactions observed, particularly with sera containing anti-EBV antibodies, were ruled out.