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Analyses of the cell mechanical damage during microinjection.

Fei Liu1, Dan Wu, Xiaoyong Wu

  • 1State Key Laboratory of Mechanical Transmission, Department of Mechanical Engineering, Chongqing University, Room 315-2, Teaching Building 7, Chongqing, 400044, China. liufei09@mit.edu.

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Summary

Microinjection can damage soft cells. Simulations and experiments show that using a small, sharp microinjector tip at high velocity minimizes mechanical damage to cells.

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Area of Science:

  • Biophysics
  • Cell biology
  • Biomechanical engineering

Background:

  • Microinjection is crucial for introducing substances into cells.
  • Cell rupture during microinjection causes mechanical damage.
  • Understanding and mitigating this damage is essential for cell-based research.

Purpose of the Study:

  • To analyze control parameters influencing mechanical damage during microinjection.
  • To develop a computational model simulating cell mechanics and damage.
  • To identify optimal microinjection parameters for reducing cell rupture.

Main Methods:

  • Developed a computational model using dissipative particle dynamics.
  • Simulated cell membrane networks, cytoskeleton, and crosslink proteins.
  • Validated the model using weak power law rheology and experimental data from Zebrafish embryos.

Main Results:

  • The computational model accurately reflects cell mechanical properties.
  • The number of ruptured bonds quantifies mechanical damage.
  • Microinjector tip size/shape and injection velocity significantly impact cell damage.

Conclusions:

  • A small, sharp microinjector tip reduces mechanical damage.
  • High injection velocity is associated with decreased cell damage.
  • Optimizing microinjection parameters is key to preserving cell integrity.