Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Control of plant organ growth linked to cell division orientation in inner tissues.

Current biology : CB·2026
Same author

Infrared Spectroelectrochemical Insights into Rhenium-Based Supramolecular Assemblies for Electron Storage and Transfer.

Inorganic chemistry·2026
Same author

Thermodynamic and Kinetic Effects of Ion Pairing in Class II/III Mixed-Valent Systems.

The journal of physical chemistry. B·2026
Same author

Tension TRAAKer: a chemigenetic fluorescent membrane tension reporter.

bioRxiv : the preprint server for biology·2026
Same author

Sialoblastoma of the Minor Salivary Glands: A Case Report.

Case reports in dentistry·2026
Same author

Proteome-Driven Phenotyping of Identified Single Neurons in Intact Brain Tissue by Aspiration Patch Proteomics.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Apr 18, 2026

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
09:57

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

Published on: February 4, 2016

11.4K

Improved PeT molecules for optically sensing voltage in neurons.

Clifford R Woodford1, E Paxon Frady, Richard S Smith

  • 1Departments of Chemistry and Biochemistry, ‡Pharmacology, §Neurosciences Graduate Group, ∥Division of Biological Sciences, ⊥Biomedical Sciences, and #Howard Hughes Medical Institute, University of California, San Diego , La Jolla, California 92093, United States.

Journal of the American Chemical Society
|January 14, 2015
PubMed
Summary

New VoltageFluor (VF) dyes offer improved optical voltage sensing in excitable cells. The lead dye, VF2.1(OMe).H, demonstrates enhanced sensitivity for studying neural circuit activity.

More Related Videos

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices
12:51

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices

Published on: November 29, 2012

17.3K
Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye
06:43

Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye

Published on: June 20, 2019

8.4K

Related Experiment Videos

Last Updated: Apr 18, 2026

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
09:57

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

Published on: February 4, 2016

11.4K
Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices
12:51

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices

Published on: November 29, 2012

17.3K
Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye
06:43

Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye

Published on: June 20, 2019

8.4K

Area of Science:

  • Neuroscience
  • Biochemistry
  • Biophysics

Background:

  • VoltageFluor (VF) dyes enable optical measurement of voltage in excitable membranes.
  • High spatial and temporal resolution is crucial for characterizing voltage dynamics in cell populations.
  • Existing VF dyes utilize photoinduced electron transfer (PeT) for voltage sensing.

Purpose of the Study:

  • To develop novel VF dyes with enhanced voltage sensitivity.
  • To correlate chemical structure modifications with voltage sensitivity.
  • To validate the performance of new VF dyes in various biological systems.

Main Methods:

  • Systematic chemical substitution to tune the PeT driving force (ΔGPeT + w).
  • Estimation of (ΔGPeT + w) values using experimentally measured redox potentials.
  • Validation of voltage sensitivities in patch-clamped HEK cells, neurons, and ganglia.
  • Application in mouse olfactory bulb slices to assess pharmacological effects and circuit activity.

Main Results:

  • Ten new VF dyes were synthesized and characterized.
  • The dye VF2.1(OMe).H exhibited a 48% ΔF/F per 100 mV sensitivity.
  • VF2.1(OMe).H showed a 2-fold improvement in sensitivity compared to previous dyes.
  • The dye successfully reported on pharmacological effects and neural circuit activity in brain slices.

Conclusions:

  • Chemical modification of VF dyes effectively modulates voltage sensitivity.
  • VF2.1(OMe).H represents a significant advancement in voltage-sensitive dye technology.
  • This new generation of VF dyes expands the possibilities for optical electrophysiology research.