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Optical Gratings Coated with Thin Si3N4 Layer for Efficient Immunosensing by Optical Waveguide Lightmode Spectroscopy
Lorena Diéguez1, David Caballero2, Josep Calderer3
1Department of Electronics, University of Barcelona, C/Martí i Franquès 1, Barcelona, ES 08028, Spain. lorena.dieguez@unisa.edu.au.
Biosensors
|January 15, 2015
Summary
New silicon nitride coatings on optical gratings show promise for real-time biosensing. This advancement in optical waveguide technology paves the way for integrating biosensors with microelectronics.
Area of Science:
- Materials Science
- Biotechnology
- Optoelectronics
Background:
- Optical gratings are crucial components in various sensing applications.
- Silicon nitride (Si3N4) is a material with potential for biosensor development.
- Real-time monitoring of biological interactions is essential for diagnostics.
Purpose of the Study:
- To develop and evaluate silicon nitride coated optical gratings for biosensing.
- To demonstrate the feasibility of using these gratings with Optical Waveguide Lightmode Spectroscopy (OWLS).
- To explore the integration of optical waveguides with microelectronics for advanced biosensors.
Main Methods:
- Deposition of a 10 nm transparent silicon nitride layer on commercial optical gratings via sputtering.
- Characterization of the silicon nitride layer using X-ray photoelectron spectroscopy and atomic force microscopy.
- Testing the sensor performance using Optical Waveguide Lightmode Spectroscopy (OWLS).
Main Results:
- The quality of the sputtered silicon nitride layer was confirmed.
- The silicon nitride coated gratings successfully functioned as sensors.
- OWLS demonstrated real-time detection of an antibody-protein pair based on concentration.
Conclusions:
- Silicon nitride is a viable functional layer for optical biosensors.
- The developed Si3N4 coated gratings enable real-time detection of biomolecular interactions.
- This technology offers new possibilities for integrating optical waveguides with microelectronics in biosensing platforms.

