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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

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Published on: February 9, 2024

DNA/RNA Detection Using DNA-Templated Few-Atom Silver Nanoclusters.

Judy M Obliosca¹, Cong Liu², Robert Austin Batson³

¹Department of Biomedical Engineering, Cockrell School of Engineering, University of Texas at Austin, Austin, TX 78712, USA. jmobliosca@utexas.edu.

|January 15, 2015

Summary

DNA-templated silver nanoclusters offer tunable fluorescence for brighter, more photostable applications. These nanomaterials enable sensitive DNA detection and single-nucleotide polymorphism identification.

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DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

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Published on: September 27, 2019

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DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

Published on: September 27, 2019

Area of Science:

Nanomaterials Science
Biotechnology
Analytical Chemistry

Background:

DNA-templated silver nanoclusters (DNA/Ag NCs) are novel organic/inorganic nanomaterials.
Their fluorescence emission is tunable across visible and near-IR spectra via sequence programming.
DNA/Ag NCs exhibit superior brightness and photostability over organic dyes and offer advantages over quantum dots (smaller, less blinking, non-toxic).

Purpose of the Study:

To review recent advancements in DNA/Ag NCs for developing DNA/RNA sensors.
To highlight the application of DNA/Ag NCs in creating novel molecular probes for DNA detection.
To discuss the potential of DNA/Ag NCs in identifying single-nucleotide polymorphisms (SNPs).

Main Methods:

Utilizing DNA sequences as templates to synthesize few-atom silver nanoclusters.
Programming template sequences to tune fluorescence emission properties.
Developing NanoCluster Beacons (NCBs) based on DNA/Ag NCs for fluorogenic and color-switching DNA detection.

Main Results:

DNA/Ag NCs demonstrate tunable fluorescence emission.
NanoCluster Beacons (NCBs) enable sensitive DNA detection and SNP differentiation by emission color.
DNA/Ag NCs exhibit environmental sensitivity, useful for various detection applications.

Conclusions:

DNA/Ag NCs represent a versatile platform for developing advanced DNA/RNA sensors.
NCBs offer an inexpensive and accessible method for DNA detection and SNP analysis.
The unique properties of DNA/Ag NCs facilitate the creation of novel biosensing tools.