Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

HM-PAO-imaging and herpes encephalitis.

G M Cleator1, A G Lewis, P E Klapper

  • 1Department of Pathological Sciences, Medical School, University of Manchester, England.

Archives of Virology
|January 1, 1989
PubMed
Summary

Researchers investigated if the brain imaging agent 99mTc-hexamethylpropyleneamine oxime (HM-PAO) is selectively taken up by cells infected with Human Herpesvirus 1 (HSV-1). The study found no specific uptake in infected rats or cells, indicating HM-PAO is not suitable for this purpose.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pharmacological targeting of C5a receptors during organ preservation improves kidney graft survival.

Clinical and experimental immunology·2008
Same author

Obtaining consent for examination and treatment.

BMJ (Clinical research ed.)·2001
Same author

Nature of immobilized antibody layers linked to thioctic acid treated gold surfaces.

Biosensors & bioelectronics·2001
Same author

Synthesis of 4'-O-acetyl-maltose and alpha-D-galactopyranosyl-(1-->4)-D-glucopyranose for biochemical studies of amylose biosynthesis.

Carbohydrate research·2001
Same author

A literature review of the consequences of prenatal marihuana exposure. An emerging theme of a deficiency in aspects of executive function.

Neurotoxicology and teratology·2001
Same author

Biodistribution of ultrasmall iron oxide particles in the rat liver.

Journal of magnetic resonance imaging : JMRI·2001

Area of Science:

  • Neuroimaging
  • Virology
  • Radiochemistry

Background:

  • Human Herpesvirus 1 (HSV-1) can cause encephalitis, a serious brain infection.
  • Cerebral blood-flow imaging agents like 99mTc-hexamethylpropyleneamine oxime (HM-PAO) are used to diagnose various neurological conditions.
  • Developing targeted imaging agents for viral infections is crucial for early diagnosis and treatment.

Purpose of the Study:

  • To determine if the cerebral blood-flow imaging agent 99mTc-hexamethylpropyleneamine oxime (HM-PAO) is selectively taken up by cells infected with Human Herpesvirus 1 (HSV-1).
  • To evaluate the potential of HM-PAO as a diagnostic tool for HSV-1 encephalitis.

Main Methods:

  • In vivo studies using encephalitic rats with brain scintigraphic imaging.
  • In vitro studies using HSV-1 infected Vero cells.

Related Experiment Videos

  • Histopathological analysis including immunoperoxidase staining and autoradiography of brain sections.

    • Main Results:

    • No specific uptake of 99mTc-hexamethylpropyleneamine oxime (HM-PAO) was observed in the brains of encephalitic rats.
    • No selective uptake of HM-PAO was detected in HSV-1 infected Vero cells.
    • Histopathological analyses confirmed the absence of targeted accumulation of the imaging agent in infected tissues.

    Conclusions:

    • 99mTc-hexamethylpropyleneamine oxime (HM-PAO) does not show selective uptake by Human Herpesvirus 1 (HSV-1) infected cells, either in vivo or in vitro.
    • HM-PAO is not a suitable agent for the specific imaging or diagnosis of HSV-1 encephalitis based on these findings.
    • Further research is needed to identify or develop novel imaging agents with specificity for viral infections of the brain.