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A low-cost, high-performance system for fluorescence lateral flow assays.

Linda G Lee1, Eric S Nordman2, Martin D Johnson3

  • 1Song Diagnostic Research LLC, 1 Megans Lane, Woodside, CA 94062, USA. Linda@songdx.net.

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Summary

We developed a sensitive fluorescence lateral flow system for quantitative detection. This inexpensive platform offers a wide dynamic range, outperforming traditional methods for point-of-care diagnostics.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Point-of-Care Diagnostics

Background:

  • Lateral flow assays are widely used for rapid diagnostics.
  • Traditional methods often lack sensitivity and dynamic range.
  • Fluorescence detection offers potential for improved performance.

Purpose of the Study:

  • To develop and validate a sensitive fluorescence lateral flow system.
  • To compare fluorescence detection with colloidal gold absorbance detection.
  • To assess the platform's suitability for point-of-care applications.

Main Methods:

  • Utilized an LED illumination system with plastic optics and filters.
  • Employed R-phycoerythrin (R-PE) as a fluorescent dye.
  • Compared fluorescence detection with colloidal gold using biotinylated BSA and hCG analytes.

Main Results:

  • Achieved excellent sensitivity and a wide dynamic range (0.4-4,000 ng/mL) with fluorescence.
  • Demonstrated a 1,000-fold signal change with fluorescence, superior to colloidal gold's 10-fold change.
  • R-PE's long Stokes shift enabled the use of cost-effective plastic filters.

Conclusions:

  • The developed fluorescence lateral flow system provides sensitive and quantitative detection.
  • This inexpensive, high-performance platform is suitable for point-of-care diagnostics.
  • Fluorescence detection offers significant advantages over absorbance-based methods in lateral flow assays.