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Updated: Apr 18, 2026

Amplicon Sequencing using the Long-Read Sequencing Technologies
Published on: August 29, 2025
Whole genome sequencing: a new paradigm in the surveillance and control of human tuberculosis
Seyed E Hasnain1, Ronan F O'Toole2, Sonam Grover3
1Kusuma School of Biological Sciences, Indian Institute of Technology (IIT), Hauz Khas, New Delhi 110016, India; Dr. Reddy's Institute of Life Sciences, University of Hyderabad Campus, Prof. C. R. Rao Road, Gachibowli, Hyderabad 500007, India.
Abstract:
Whole Genome Sequencing (WGS) is emerging as a very powerful tool for the management, outbreak analyses, surveillance and determining drug resistance of human infectious pathogens including Mycobacterium tuberculosis and MRSA. WGS can also discriminate relapse TB from re-infection and the resolution provided by WGS has no comparison to conventional technologies. With current cost coming down to <£70 per bacterial genome, WGS has emerged as an alternative to all the existing technologies put together. We discuss the advantage and disadvantages of WGS and whether it can become a point of care tool in not just developed countries but also in developing countries which have a huge TB burden. The likely utility of WGS for other pathogens and also in characterizing holobionts is also discussed.
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