Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Serum Biomarkers of Macrophage Activation and Incident CKD in People With and Without HIV: Findings From the MACS-WIHS Combined Cohort Study.

Open forum infectious diseases·2026
Same author

Role of biomarkers in predicting disease severity in acute dengue and SARs-CoV-2-Infected patients.

BMC infectious diseases·2025
Same author

Effect of Pitavastatin on Epigenetic Aging Biomarkers in People With HIV: Pilot Substudy of the REPRIEVE Trial.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2025
Same author

Glycation metabolites predict incident age-related comorbidities and mortality in older people with HIV.

GeroScience·2025
Same author

Opposite Roles of IL-32α Versus IL-32β/γ Isoforms in Promoting Monocyte-Derived Osteoblast/Osteoclast Differentiation and Vascular Calcification in People with HIV.

Cells·2025
Same author

Piloting an event-based surveillance model in private hospitals for early detection of disease clusters, Kerala, India.

The Indian journal of medical research·2025

Related Experiment Video

Updated: Apr 18, 2026

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

3.9K

HIV-associated mucosal gene expression: region-specific alterations.

Robin M Voigt1, Ali Keshavarzian, John Losurdo

  • 1aDepartment of Internal Medicine, Division of Gastroenterology, Hepatology, and Nutrition bDepartment of Pharmacology cDepartment of Physiology, Rush University Medical Center, Chicago, Illinois dDivision of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands eDepartment of Immunology/Microbiology, Rush University Medical Center, Chicago, Illinois, USA fDepartment of Biochemistry, Rush University Medical Center gRuth M. Rothstein CORE Center/Department of Medicine, Stroger Hospital of Cook County, Chicago, Illinois, USA.

AIDS (London, England)
|January 15, 2015
PubMed
Summary

Even with controlled HIV, persistent immune activation and inflammation occur. Intestinal gene expression changes in HIV-infected individuals may drive this inflammation and gut barrier issues.

More Related Videos

A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses
14:23

A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses

Published on: August 31, 2014

16.4K
Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
13:13

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

Published on: September 25, 2018

11.2K

Related Experiment Videos

Last Updated: Apr 18, 2026

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

3.9K
A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses
14:23

A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses

Published on: August 31, 2014

16.4K
Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
13:13

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

Published on: September 25, 2018

11.2K

Area of Science:

  • Immunology
  • Gastroenterology
  • Virology

Background:

  • Highly Active Antiretroviral Therapy (HAART) controls HIV but doesn't resolve systemic immune activation and inflammation.
  • Persistent inflammation contributes to non-AIDS complications in HIV-infected individuals.
  • The intestine is a key site for immune function and a source of chronic inflammation.

Purpose of the Study:

  • To investigate alterations in intestinal gene expression in individuals with virally controlled HIV.
  • To determine if intestinal gene expression changes correlate with systemic inflammation and gut barrier dysfunction.

Main Methods:

  • Gene expression profiling using Affymetrix arrays on ileum and colon biopsy samples from HIV-uninfected and HIV-infected individuals.
  • Pathway analysis of gene expression data.
  • Correlation of intestinal gene expression with systemic markers of inflammation, barrier dysfunction, and gut microbiota composition.

Main Results:

  • Upregulation of genes related to immune response, cytokine signaling, apoptosis, and cell proliferation was observed in the intestine of HIV-infected individuals.
  • Ileal gene expression correlated with systemic inflammation markers (e.g., TNF).
  • Colonic gene expression correlated with gut microbiota composition (e.g., Bacteroides).

Conclusions:

  • Virally suppressed HIV infection is associated with persistent, pro-inflammatory changes in the intestinal mucosa.
  • These intestinal gene expression alterations may contribute to or result from gut barrier dysfunction and dysbiosis in HIV.
  • Understanding these mechanisms is crucial for managing non-AIDS events in HIV patients.