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Related Concept Videos

Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

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Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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Affinity Chromatography01:03

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Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Types Of Column Chromatography01:29

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The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
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Principles Of Column Chromatography01:13

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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Chromatography: Introduction01:10

Chromatography: Introduction

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Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
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High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
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Related Experiment Video

Updated: Apr 18, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
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Functional chromatographic technique for natural product isolation.

Eric C Lau1, Damian J Mason, Nicole Eichhorst

  • 1College of Pharmacy, Department of Pharmacology and Toxicology, University of Arizona, Tucson, AZ 85721-0207, USA. chapman@pharmacy.arizona.edu.

Organic & Biomolecular Chemistry
|January 15, 2015
PubMed
Summary

Functional chromatography isolates natural products by using biological affinity as the matrix. This innovative technique directly links compound isolation with biological activity, advancing natural product drug discovery.

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Area of Science:

  • Natural Product Chemistry
  • Biochemistry
  • Drug Discovery

Background:

  • Natural product discovery relies on purification and biological assays.
  • Current methods often isolate compounds without knowing their biological action.
  • This gap hinders efficient identification of bioactive natural products.

Purpose of the Study:

  • To introduce a novel technique called functional chromatography.
  • To enable the isolation of natural products based on their biological affinity.
  • To directly link compound purification with biological function.

Main Methods:

  • Functional chromatography utilizes biological affinity as the separation matrix.
  • Compounds are isolated based on their specific interaction with the biological matrix.
  • This method integrates purification and biological assessment.

Main Results:

  • Demonstrates a method for isolating natural products with defined biological action.
  • Functional chromatography allows for targeted isolation of bioactive compounds.
  • Provides a more direct route to identifying potential drug leads from natural sources.

Conclusions:

  • Functional chromatography represents a significant advancement in natural product isolation.
  • This technique streamlines the discovery of bioactive natural products.
  • It offers a powerful tool for accelerating drug discovery pipelines.