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PCR-based specific techniques used for detecting the most important pathogens on strawberry: a systematic review.

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This review summarizes polymerase chain reaction (PCR) methods for detecting major strawberry pathogens. Effective DNA-based assays are available for rapid, reliable plant disease monitoring.

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Area of Science:

  • Plant Pathology
  • Molecular Biology
  • Agricultural Science

Background:

  • Strawberry diseases significantly limit crop yield and performance.
  • Traditional pathogen detection methods have limitations.
  • DNA-based tests, like PCR, offer sensitive and specific pathogen identification.

Purpose of the Study:

  • To review polymerase chain reaction (PCR)-based methods for detecting widespread strawberry pathogens.
  • To provide a comprehensive list of PCR protocols for pathogens like Fusarium oxysporum, Phytophthora fragariae, Colletotrichum acutatum, Verticillium dahliae, Botrytis cinerea, Macrophomina phaseolina, and Xanthomonas fragariae.
  • To aid diagnostic laboratories in developing rapid, cost-effective, and reliable monitoring techniques.

Main Methods:

  • Systematic review of PCR-based techniques for strawberry pathogen detection and quantification.
  • Searched multiple scientific databases (AGRICOLA, Scopus, Web of Knowledge, etc.) up to April 2014.
  • Included studies investigating specific strawberry pathogens using PCR and reporting pre-analytical requirements.

Main Results:

  • 22 full-text articles met inclusion criteria from 259 reviewed.
  • Identified numerous PCR protocols: 10 for X. fragariae, 8 for P. fragariae, 6 for C. acutatum, 4 for B. cinerea, 3 for V. dahliae, and 1 for F. oxysporum.
  • Highlighted accuracy and sensitivity of PCR methods but noted pre-analytical errors impact laboratory results.

Conclusions:

  • Effective PCR-based detection assays for major strawberry pathogens have been developed.
  • These assays provide a foundation for clinical labs, regulatory personnel, and diagnosticians.
  • Implementation of these methods can improve strawberry disease management.