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Temperate bacteriophages collected by outer membrane vesicles in Komagataeibacter intermedius
Alla Kharina1, Olga Podolich, Iuliia Faidiuk
1Institute of Biology of Taras Shevchenko National University of Kyiv, Kyiv, Ukraine.
Journal of Basic Microbiology
|January 16, 2015
Summary
Researchers identified novel tailed bacteriophages (Myoviridae) in cellulose-producing bacteria Komagataeibacter intermedius. These phages interact with outer membrane vesicles, suggesting a unique defense mechanism against phage spread.
Area of Science:
- Microbiology
- Bacteriology
- Virology
Background:
- Acetic acid bacteria are crucial for bacterial cellulose production and fermented products.
- Komagataeibacter intermedius is a key cellulose-producing bacterial strain.
Purpose of the Study:
- To identify temperate bacteriophages in the cellulose-producing strain Komagataeibacter intermedius IMBG180.
- To investigate the interaction between induced phages and outer membrane vesicles (OMVs).
Main Methods:
- Prophage induction using mitomycin C and nalidixic acid.
- Transmission electron microscopy (TEM) for phage visualization.
- PCR assay targeting the myovirus capsid gene for phylogenetic analysis.
Main Results:
- TEM revealed tailed bacteriophages belonging to the Myoviridae family.
- PCR confirmed the phylogenetic position of the induced phages.
- Nalidixic acid induced outer membrane vesicle (OMV)-like particles (30-80 nm and 120-200 nm) containing phage tails.
- OMVs acted as collectors for phage particles, utilizing outer membrane receptors for phage detection.
Conclusions:
- This study describes the first myoviruses affiliated with Komagataeibacter intermedius.
- Outer membrane vesicles play a role in collecting phages, potentially preventing their spread.
- This interaction suggests a novel host defense mechanism against bacteriophage infection.
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