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Updated: Apr 18, 2026
Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
Published on: July 23, 2015
New mass-spectrometry-compatible degradable surfactant for tissue proteomics
Ying-Hua Chang1, Zachery R Gregorich, Albert J Chen
1Department of Cell and Regenerative Biology, ‡Molecular and Cellular Pharmacology Program, §Department of Chemistry, ∥Human Proteomics Program, and ⊥Molecular and Environmental Toxicology Program, University of Wisconsin-Madison , 1300 University Avenue, Madison 53706, Wisconsin, United States.
A novel MS-compatible surfactant, MaSDeS, enhances tissue proteomics by improving protein solubility and identification, especially for membrane proteins. Its degradable and thermostable properties advance biomarker discovery and disease research.
Area of Science:
- Proteomics
- Biomarker Discovery
- Mass Spectrometry
Background:
- Protein solubility is a major challenge in mass spectrometry (MS)-based tissue proteomics.
- Conventional surfactants like SDS are incompatible with MS.
- Improved protein solubilization is crucial for advancing tissue proteomics.
Purpose of the Study:
- To discover an MS-compatible surfactant for tissue proteomics.
- To overcome protein solubility limitations in tissue sample preparation.
- To enhance protein identification and biomarker discovery in complex tissues.
Main Methods:
- Screening a library of surfactant-like compounds.
- Evaluating surfactant performance in tissue extraction and protein solubilization.
- Assessing compatibility with mass spectrometry (MS).
Main Results:
- Discovery of MaSDeS, an MS-compatible, acid-degradable surfactant.
- MaSDeS exhibits performance comparable to SDS in solubilizing all protein categories.
- Significantly increased protein identification in heart, liver, and lung tissues.
- Enrichment of under-represented membrane proteins.
- Demonstrated superior performance over other MS-compatible surfactants.
Conclusions:
- MaSDeS significantly advances tissue proteomics by enabling efficient protein extraction and solubilization.
- Its properties facilitate high-throughput and temperature-dependent MS-based proteomics.
- MaSDeS holds great potential for basic biomedical research and clinical applications.
- The surfactant can be widely used in various proteomics and biochemical experiments.
