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Updated: Apr 18, 2026

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies
Published on: February 23, 2018
Requirement for Fc effector mechanisms in the APOBEC3/Rfv3-dependent neutralizing antibody response
Kalani Halemano1, Bradley S Barrett2, Karl J Heilman2
1Department of Medicine, University of Colorado Denver, Aurora, Colorado, USA Department of Immunology and Microbiology, University of Colorado Denver, Aurora, Colorado, USA.
Abstract:
Antiretroviral neutralizing antibody (NAb) responses are often evaluated in the absence of Fc-dependent immune effectors. In murine Friend retrovirus infection, Apobec3/Rfv3 promotes a potent polyclonal NAb response. Here, we show that the Apobec3/Rfv3-dependent NAb response correlated with virus-specific IgG2 titers and that the in vivo neutralization potency of Apobec3/Rfv3-resistant antisera was dependent on activating Fcγ receptors but not complement. The data strengthen retroviral vaccine strategies aimed at eliciting NAbs that activate specific Fcγ receptors.
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