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Transcript changes in Vibrio cholerae in response to salt stress.

Xiuping Fu1, Weili Liang1, Pengcheng Du1

  • 1State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 155, Changbai Road, Changping, Beijing 102206 China ; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, 310006 China.

Gut Pathogens
|January 16, 2015
PubMed
Summary

Vibrio cholerae exhibits varying salt tolerance, utilizing common bacterial stress responses and specific sigma factors. High salt tolerance in these strains correlates with elevated gene transcript levels, including those for peptidoglycan biosynthesis.

Keywords:
PCR arraySalt stressTranscriptionVibrio cholerae

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Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Environmental Microbiology

Background:

  • Vibrio cholerae is a significant human intestinal pathogen.
  • It thrives in estuarine environments, facing osmotic challenges.
  • V. cholerae requires self-regulation to manage high salt stress in water and food.

Purpose of the Study:

  • To investigate the salt tolerance of V. cholerae O1 and O139 strains.
  • To elucidate the molecular mechanisms underlying V. cholerae's response to high salt stress.
  • To identify genes and pathways involved in salt resistance.

Main Methods:

  • Selection of V. cholerae O1 and O139 strains from diverse regions and years.
  • Analysis of salt tolerance across varying salt concentrations (4%-6%).
  • Gene expression analysis focusing on salt stress-response related genes, including sigma factors and those involved in ion transport and biosynthesis.

Main Results:

  • V. cholerae strains demonstrated salt resistance between 4% and 6% salt concentrations.
  • No significant difference in salt tolerance was observed between O1 and O139 groups.
  • Common bacterial salt stress responses (Na+ exclusion, K+ uptake, glutamate biosynthesis) were identified.
  • Upregulation of sigma factors and strain-specific gene expression changes were noted.
  • Hierarchical clustering revealed a correlation between gene transcript levels and salt tolerance.

Conclusions:

  • V. cholerae employs both common and potentially unique sigma factors for salt stress response.
  • Elevated transcript levels of genes, including those for sigma factors and peptidoglycan biosynthesis, are linked to higher salt tolerance.
  • Strain-specific gene expression patterns contribute to the adaptation of V. cholerae to high salt environments.