Related Experiment Videos
Acquisition and retention of viruses by Trichomonas vaginalis
F F Pindak1, M Mora de Pindak, B M Hyde
1Department of Pathology, University of South Alabama, College of Medicine, Mobile 36617.
Abstract:
Recently described occurrence of virus-like particles (VLP) in some strains of Trichomonas vaginalis suggests the possibility that the pathogenic significance of this organism may be broadened by its potential for viral transmission. Inasmuch as neither the source nor the host range of the VLP are known, any hazard which they may present for man cannot be estimated. A model has been established for the study of acquisition of known human viruses by T vaginalis. Tissue cultures were infected with two reoviruses and a fresh isolate of genital herpes simplex virus (HSV). A squirrel monkey reovirus was also included in the study. T vaginalis was inoculated into the virus cultures three days later. The progress of virus acquisition by the trichomonads was monitored by transmission electron microscopy and by culture. Virus-containing cell fragments were engulfed by trichomonads and internalised in vacuoles. After digestion of cellular debris only virus particle aggregates were retained. Viable reoviruses were recovered from the trichomonads for nine days, and HSV for six days. The results suggest the possibility of transmission of at least some viruses by T vaginalis.
Insights
Trichomonas vaginalis may transmit viruses. This study shows the parasite can acquire and retain human reoviruses and herpes simplex virus (HSV) in laboratory models.
Area of Science:
- Microbiology
- Virology
- Parasitology
Background:
- Virus-like particles (VLPs) in Trichomonas vaginalis suggest potential viral transmission.
- The source and host range of VLPs are unknown, limiting hazard assessment.
Purpose of the Study:
- To establish a model for studying the acquisition of human viruses by T. vaginalis.
- To investigate the potential for T. vaginalis to act as a vector for viral transmission.
Main Methods:
- Tissue cultures infected with reoviruses and herpes simplex virus (HSV).
- T. vaginalis inoculated into virus cultures and monitored via electron microscopy and culturing.
- Analysis of virus acquisition, internalization, and retention by T. vaginalis.
Main Results:
- T. vaginalis engulfed virus-containing cell fragments and retained virus particle aggregates.
- Viable reoviruses were recovered from T. vaginalis for up to nine days.
- Viable HSV was recovered from T. vaginalis for up to six days.
Conclusions:
- T. vaginalis can acquire and retain human viruses, including reoviruses and HSV.
- The findings suggest T. vaginalis may transmit certain viruses.
- Further research is needed to assess the public health implications of this potential transmission route.