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Updated: Apr 18, 2026

Phenotyping Mouse Pulmonary Function In Vivo with the Lung Diffusing Capacity

Phenotyping Mouse Pulmonary Function In Vivo with the Lung Diffusing Capacity

Published on: January 6, 2015

Phenotyping mouse pulmonary function in vivo with the lung diffusing capacity.

Nathachit Limjunyawong¹, Jonathan Fallica¹, Amritha Ramakrishnan²

¹Department of Environmental Health Sciences, Johns Hopkins University Bloomberg School of Public Health.

Journal of Visualized Experiments : Jove

|January 16, 2015

Summary

This summary is machine-generated.

A new method quantifies diffusing capacity for carbon monoxide in mice, mirroring human lung function tests. This technique aids in studying lung diseases and development in mouse models.

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Last Updated: Apr 18, 2026

Phenotyping Mouse Pulmonary Function In Vivo with the Lung Diffusing Capacity

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Published on: January 6, 2015

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Area of Science:

Pulmonary Medicine
Animal Models
Respiratory Physiology

Background:

Mice are crucial for modeling human lung diseases.
Quantifying lung pathology requires translational phenotypic methods.
Few existing mouse lung function tests are applicable to humans.

Purpose of the Study:

To develop a simple, quick method for measuring diffusing capacity for carbon monoxide (DLCO) in mice.
To establish a pulmonary function test with translational relevance to human diagnostics.
To validate the method's ability to detect lung pathologies and developmental changes.

Main Methods:

Brief lung inflation with tracer gases in anesthetized mice.
1-minute gas analysis following inflation.
Application in various induced lung pathologies and developmental studies.

Main Results:

The method successfully measured DLCO in mice.
Significant decreases in DLCO were observed in models of emphysema, fibrosis, acute lung injury, and infections.
Increasing DLCO correlated with lung maturation in young pups.

Conclusions:

This DLCO measurement in mice offers a valuable pulmonary function test.
It enables detection of structural lung changes across diverse pathologies and developmental stages.
The method enhances the translational relevance of mouse models for lung disease research.