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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Published on: February 16, 2018
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Redox-tagged peptide for capacitive diagnostic assays
Adriano Santos1, Julia P Piccoli1, Norival A Santos-Filho1
1Department of Physical Chemistry, Chemistry Institute, São Paulo State University (UNESP, Universidade Estadual Paulista), Nanobionics Research Group, 55 Prof. Francisco Degni Street, 14800-060 Araraquara, São Paulo, Brazil(2).
Biosensors & Bioelectronics
|January 16, 2015
Summary
Researchers developed a novel ferrocene redox-tagged peptide for electrochemical biosensors. This self-assembling peptide creates alternative surfaces for detecting biomarkers like C-reactive protein (CRP) at the point-of-care.
Area of Science:
- Electrochemistry
- Biosensor Technology
- Biomarker Detection
Background:
- Early disease detection relies on effective assays.
- Redox capacitive biosensors offer potential for point-of-care diagnostics.
- Need for alternative surfaces to capture clinical biomarkers in biosensing platforms.
Purpose of the Study:
- To develop alternative receptive surfaces for novel electrochemical biosensing platforms.
- To synthesize a ferrocene redox-tagged peptide for self-assembly onto metallic interfaces.
- To functionalize biological surfaces for bedside diagnostic assays.
Main Methods:
- Synthesis of a ferrocene redox-tagged peptide.
- Self-assembly of the peptide onto metallic interfaces.
- Utilized C-reactive protein (CRP) as a model biomarker for proof of concept.
- Compared results with previously reported capacitive assays.
Main Results:
- Achieved a limit of detection of 0.8 nmol L(-1) for CRP.
- Demonstrated a linear range (R(2)∼98%) from 0.5-10.0 nmol L(-1) with the logarithm of the analyte concentration.
- The results fall within the clinically relevant range for CRP detection.
Conclusions:
- The ferrocene redox-tagged peptide approach provides a viable alternative surface for electrochemical biosensing.
- This method is suitable for recruiting clinical biomarkers like CRP.
- The developed functionalization holds promise for point-of-care diagnostic applications.
