[Effect of compound hypertonic saline solution on septic rats].

Area of Science:

• Sepsis Research
• Resuscitation Fluids
• Critical Care Medicine

Background:

• Sepsis is a life-threatening condition characterized by dysregulated host response to infection.
• Acute lung injury (ALI) and circulatory failure are common complications of sepsis.
• Effective resuscitation strategies are crucial for improving outcomes in sepsis patients.

Purpose of the Study:

• To investigate the therapeutic effects of compound hypertonic saline solution (HSD) in a rat model of sepsis.
• To evaluate the impact of HSD on survival rates, hemodynamic parameters, inflammatory markers, and lung injury in sepsis.

Main Methods:

• A cecal ligation and puncture (CLP) model was used to induce sepsis in male Wistar rats.
• Rats were divided into four groups: sham operation, CLP, CLP + normal saline (NS), and CLP + HSD.
• Survival rates, mean arterial pressure (MAP), plasma cytokine levels (TNF-α, IL-1β, PCT), and lung injury markers (neutrophil count, MPO activity, W/D ratio) were assessed.

Main Results:

• HSD administration significantly increased survival rates at 9 and 18 hours post-CLP compared to CLP and CLP + NS groups (P<0.05 or P<0.01).
• HSD improved MAP and reduced elevated plasma levels of TNF-α, IL-1β, and PCT at 9 and 18 hours.
• HSD decreased neutrophil infiltration, MPO activity, and lung W/D ratio, alleviating pathological lung injury.

Conclusions:

• Compound hypertonic saline solution (HSD) demonstrates significant therapeutic benefits in a rat model of sepsis.
• HSD effectively ameliorates circulatory failure, reduces systemic inflammation, and mitigates acute lung injury.
• HSD improves the overall survival rate in septic rats, suggesting its potential as a resuscitation fluid in sepsis management.